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KMID : 0364819990350040258
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Korean Journal of Microbiology
1999 Volume.35 No. 4 p.258 ~ p.265
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A Specific Role of Ime2, Meiosis-specific Protein Kinase, in the Early Meiotic Pathway in Saccharomyces cerevisiae
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Abstract
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Entry into meiosis in the yeast Saccharomyces cerevisiae is regulated by two major factors: the cell type (MATa/MATα) and the nutriational state (starvation) of the cell. The two independent regulations act through IME1 and IME2 expression to initiate meiosis. IME2 encodes a meiosis-specific protein kinase, and it enabled MATa/MATα diploid cells to undergo meiosis and sporulation. The PCR mutagenesis method was applied for the isolation of thermosensitive ime2 mutants. Among sixty tow mutants isolated from the phenotype of defective spore formation under the restrictive temperature, three with the most easily observed temperature-sensitive phenotype (tsㆍ
ime2-11, tsㆍime2-12 and tsㆍime2-13) were selected for further study. To understand the detailed functions of IME2, we examined the defects of these mutants in the early meiotic pathway including the premeiotic DNA replication and meiotic recombination. At the restrictive temperature, the ime2 mutants were defective in premeiotic DNA replication and exhibited decreased level in meiotic recombination. These results suggest that the IME2 gene plays essential role in meiotic recombination pathway as well as premeiotic DNA replication. As the result of the IME2 overexpression in mre4, moreover, it was suggested that the IME2 and MRE4 genes act on the same pathway of initiation step in meiotic recombination.
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KEYWORD
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IME2, meiotic recombination, S. cerevisiae, spore formation,
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